Journal: Cell host & microbe

Article Title: Longitudinal human antibody repertoire against complete viral proteome from Ebola virus survivor reveals protective sites for vaccine design

doi: 10.1016/j.chom.2020.01.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Zaire EBOV VP40 Matrix protein , Sino Biologicals , 40446-V07E.

Techniques: Recombinant

Journal: Cell Discovery

Article Title: Structural basis of a two-antibody cocktail exhibiting highly potent and broadly neutralizing activities against SARS-CoV-2 variants including diverse Omicron sublineages

doi: 10.1038/s41421-022-00449-4

Figure Lengend Snippet: a Binding profiles of F61 and D2 to different spike proteins (WIV04-S1, WIV04-RBD, WIV04-NTD, Delta-S1, Delta-RBD and Omicron-RBD) were determined by ELISA. F61 and D2 bound to diverse RBD and S1 fragments but not WIV04-NTD with EC 50 values less than 4.5 ng/mL, indicating that they were both RBD-specific antibodies. Experiments were performed in duplicate, n = 2. Data are represented as means ± SD (upper panel) and means (down panel). b Binding kinetics ( K D ) of the monoclonal antibodies (mAbs) with Delta-RBD, Omicron-RBD (BA.1 and BA.2) were measured by SPR. c mAbs blocked SARS-CoV-2 WT-RBD binding to ACE2 measured by FACS. The X-axis represented the fluorescence intensity of human antibodies labeled by FITC, and the Y-axis represented the fluorescence intensity of RBD-mFc labeled by Taxes Red. Percentages of cells that scored negative, single positive, or double positive are shown in each quadrant.

Article Snippet: ELISA plates were coated with SARS-CoV-2 protein including WT-S1, WT-NTD, WT-RBD, Delta-S1, Delta-RBD and Omicron-RBD (Sino Biological, China) at 4 °C overnight.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Fluorescence, Labeling

Journal: Cell Discovery

Article Title: Structural basis of a two-antibody cocktail exhibiting highly potent and broadly neutralizing activities against SARS-CoV-2 variants including diverse Omicron sublineages

doi: 10.1038/s41421-022-00449-4

Figure Lengend Snippet: a Overall structure of SARS-CoV-2 S in complex with F61 Fab. The tilt angle of RBD is defined by the angle between the long axis of RBD (red line) and its projection on the horizontal plane (black ellipse). Angle between them is indicated. SARS-CoV-2 RBD is colored in cyan, other domains in gray, heavy chain of F61 in magenta and light chain of F61 in violet. Related to Supplementary Fig. . b Overall structure of SARS-CoV-2 S in complex with D2 Fab. The tilt angle of RBD is defined by the angle between the long axis of RBD (red line) and its projection on the horizontal plane (black ellipse). Angle between them is indicated. SARS-CoV-2 RBD is colored in cyan, other domains in gray, heavy chain of D2 in orange and light chain of D2 in light orange. Related to Supplementary Fig. . c Overall structures of SARS-CoV-2 Omicron S in complex with F61 Fab and D2 Fab. Color schemes are the same as a and b . Related to Supplementary Fig. . d Structural superposition of RBD-fab and RBD-ACE2 (PDB ID: 6M0J) structures and the footprints of F61, D2 and ACE2 on the RBD, and structure of Omicron-RBD-F61-D2 with footprints of F61 and D2 on the RBD. ACE2 is colored in blue. The footprints of F61, D2 and ACE2 are represented as magenta, orange and blue, respectively. Other color schemes are the same as a and b .

Article Snippet: ELISA plates were coated with SARS-CoV-2 protein including WT-S1, WT-NTD, WT-RBD, Delta-S1, Delta-RBD and Omicron-RBD (Sino Biological, China) at 4 °C overnight.

Techniques:

Journal: Scientific Reports

Article Title: Antibody-dependent-cellular-cytotoxicity-inducing antibodies significantly affect the post-exposure treatment of Ebola virus infection

doi: 10.1038/srep45552

Figure Lengend Snippet: ( A ) Four murine anti-EBOV mAbs, M001, M401, M318, and M501, were injected to evaluate their prevention efficacies (upper) and treatment efficacies (lower). The mAbs were injected at 3 days or 4 h before the inoculation of pHIV–ZGP–Fluc to determine their preventive efficacies, and at 12 h or 3 days after viral inoculation to determine their treatment efficacies. Photon flux was measured at 4 dpi. The first column shows the control pHIV–ZGP–Fluc-infected BALB/c mice that did not receive any mAb treatment. ( B – C ) Preventive efficacy ( B ) and treatment efficacy ( C ) were detected as the total photon flux in pHIV–ZGP–Fluc-infected mice treated with various mAbs compared with that in similarly infected mice without mAb treatment (first column). * p < 0.05; ** p < 0.01. ( D – I ) ELISA binding curves for recombinant GP proteins, including recombinant GP ( D ) and its receptor-binding domain ( E ) from EBOV strain H.sapiens-wt/GIN/2014/Kissidougou-C15 and recombinant GP ( F ), receptor-binding domain ( G ), GP2 ( H ), and GP1 ( I ) from EBOV strain Mayinga 1976.

Article Snippet: A 6-week-old female BALB/c mouse was immunized by an IP injection with 5 μg of a recombinant GP1 protein (40442-V08B) from EBOV (strainH.sapiens-wt/GIN/2014/Kissidougou- C15) and recombinant GP protein (40304-V08B1) from subtype Zaire, strain Mayinga 1976 (Sinobiological Inc., Beijing, China).

Techniques: Injection, Infection, Enzyme-linked Immunosorbent Assay, Binding Assay, Recombinant